DNA-nanosensor diagnostics

External consultant - Dmitry Kolpashchikov.

DNA nanosensors are specifically selected fragments of oligonucleotides capable of highly selective binding to a nucleic acid site of interest and generating a detectable signal.

The group uses two variants of DNA nanosensors: deoxyribozyme, for a fluorescent signal, (Fig. 1a) and sensors with a G-quadruplex, which create a colorimetric signal (Fig. 1b).

 

Рис. 1. Дизайн дезоксирибозима: а) с флуорофором на одном конце (F), создающий флуоресцентный сигнал (1); б) с G-квадруплексом, создающий колориметрический сигнал (2).

Fig. 1. Deoxyribozyme design: a) with a fluorophore at 5’ (F), creating a fluorescent signal (1); b) with a G-quadruplex, creating a colorimetric signal (2).

The use of DNA nanosensors has advantages over existing common diagnostic methods. In particular, DNA nanosensors are more sensitive to single nucleotide substitutions and do not require the use of expensive devices and protein reagents. This reduces the cost of production, simplifies storage and allows the use of DNA nanosensors in conditions of limited funding or in testing devices at the point of care.

The final aim of the laboratory is to create devices that will be used for the point of care diagnostics and will combine the advantages of PCR and test strips.

 

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