The emergence of hypervirulent blaNDM-1-positive Klebsiella pneumoniae sequence type 395 in an oncology hospital

Авторы: Irina Lazareva, Vladimir Ageevets, Julia Sopova, Marina Lebedeva, Старкова П.С., Daria Likholetova, Maria Lebedeva, Vladimir Gostev, Vladimir Moiseenko, Vitaliy Egorenkov, Arina Navatskaya, Galina Mitroshina, Elena Myasnikova, Irina Tsvetkova, Yuri Lobzin, Sergey Sidorenko

Abstract

Fifteen hypermucoviscous isolates (13 bla_NDM-1-positive) obtained from 11 oncology patients were analyzed by whole-genome sequencing, and selected isolates were assessed in a murine model of sepsis. ST395/K2 isolates harboring rmpA, rmpA2, peg-344, aerobactin, enterobactin, yersiniabactin, type I fimbriae, etc. displayed maximal virulence in the mouse lethality assay (LD₅₀ = 10² CFU). ST147/K20 isolates lacking yersiniabactins were relatively less virulent (LD₅₀ = 10⁴ CFU), ST395/K2 isolates lacking rmpA, rmpA2, peg-344, and aerobactin, but harboring yersiniabactin demonstrated minimal virulence (LD₅₀ = 10⁵ CFU). Isolates represent various paths and stages of evolution directed towards convergence of multidrugresistant classical Klebsiella pneumoniae and hypervirulent K. pneumoniae.

Keywords

Antimicrobial resistance, Virulence, New Delhi metallo-beta-lactamase, Hypermucoviscous Klebsiella pneumoniae

https://doi.org/10.1016/j.meegid.2020.104527

Read Full: https://www.sciencedirect.com/science/article/pii/S1567134820303580