Authors: Irina Lazareva, Vladimir Ageevets, Julia Sopova, Marina Lebedeva, Starkova P.S., Daria Likholetova, Maria Lebedeva, Vladimir Gostev, Vladimir Moiseenko, Vitaliy Egorenkov, Arina Navatskaya, Galina Mitroshina, Elena Myasnikova, Irina Tsvetkova, Yuri Lobzin, Sergey Sidorenko
Fifteen hypermucoviscous isolates (13 blaNDM-1-positive) obtained from 11 oncology patients were analyzed by whole-genome sequencing, and selected isolates were assessed in a murine model of sepsis. ST395/K2 isolates harboring rmpA, rmpA2, peg-344, aerobactin, enterobactin, yersiniabactin, type I fimbriae, etc. displayed maximal virulence in the mouse lethality assay (LD50 = 102 CFU). ST147/K20 isolates lacking yersiniabactins were relatively less virulent (LD50 = 104 CFU), ST395/K2 isolates lacking rmpA, rmpA2, peg-344, and aerobactin, but harboring yersiniabactin demonstrated minimal virulence (LD50 = 105 CFU). Isolates represent various paths and stages of evolution directed towards convergence of multidrugresistant classical Klebsiella pneumoniae and hypervirulent K. pneumoniae.
Antimicrobial resistance, Virulence, New Delhi metallo-beta-lactamase, Hypermucoviscous Klebsiella pneumoniae