A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids

Авторы: Kamar O., Sun S.-C., Lin C.-H., Chung W.-Y. , Lee M.-S., Liao Y.-C., Колпащиков Д.М., Chuang M.-C.

Abstract

Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases.

DOI: 10.1039/C7CC05576E.

Read Fullhttp://pubs.rsc.org/en/Content/ArticleLanding/2017/CC/C7CC05576E#!divAbstract.