Vladimir Brukhin

Worked as a Leading Researcher at the Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University from 2013 to 2020 right after returning from the UK, where he worked as a Senior Research Fellow at the School of Biology, University of Edinburgh. His main research interests are in the field of functional genetics, comparative genomics, epigenetics, sexual and asexual plant reproduction, developmental biology, and evolution.

At St. Petersburg State University Dr. Brukhin coordinated the Genome Russia project, the main goal of which is the creation of a web-based database containing depersonalized information on the whole-genome sequences of at least 2,500 men and women originating from the different regions of Russia, whose ancestors were indigenous to the region for several generations, as well as a description of the genome variations in these groups, detection of the features that affect the spread of diseases and creation of a database of medically-relevant genomic variants characteristic to the Russian population. He also supervised plant genomic projects within the Swiss-Russian and Indian-Russian collaboration programs “. The main goal of both projects was a better understanding of the molecular basis of apomixis the asexual reproduction of plants through seed, that could become a key ‘enabling technology’ of immense benefit to agriculture because it would enable the permanent fixation of heterosis in crop plants. Also, Dr. Brukhin developed a project “Whole-genome methylation analysis in species-specific phenotypes and at the various developmental stages”.

Dr. Brukhin received his Master in Agricultural Chemistry in 1988 from the faculty of Biology and Soil Science, St. Petersburg State University. In 1993 he earned a Ph.D. in Developmental Biology from Komarov Botanical Institute, Russian Academy of Sciences. In 1994 – Dr. Brukhin worked as a trainee at the University of Lublin in Poland where he studied plant developmental biology and microscopy. In 1997-1999 He was a postdoctoral researcher in France and Sweden studying somatic embryogenesis, apoptosis and genes specifically expressed during and after fertilization. In 2000-2006 he worked as a Senior Research Associate at the Department of Plant Developmental Genetics, University of Zurich, Switzerland, where he was responsible for coordinating the lab contributions to the multinational EU project EXOTIC (Exon Trapping Insert Consortium) and also he developed an independent research project ‘The Ubiquitin 26S Proteasome Proteolytic Pathway and Plant Reproduction’. At the University of Zurich, Dr. Brukhin was a practical instructor for Mittlebaukurs, Plant Developmental Biology. In 2006-2007 he was invited to the University of Illinois at Urbana-Champaign to participate in the project on developmental aspects of posttranslational protein modifications. In 2008-2013 Dr. Brukhin worked in the UK (the University of Aberystwyth and University of Edinburgh) where he studied epigenetics, problems of plant cell identity, and specialization. In the UK he was also involved intuition and supervision of undergraduate and PhD students.

List of publications:

Brukhin V. (2020) Epigenetic Control in Plants. Epigenomes 4(3), 11; https://doi.org/10.3390/epigenomes4030011

Zhernakova D.V., Brukhin V., Malov S., Oleksyk T.K., Koepfli K.P., Zhuk A., Dobrynin P., Kliver S., Cherkasov N., ..., O'Brien S.J. et al. (2019) Genome-wide sequence analyses of ethnic populations across Russia. Genomics. 2020 Jan;112 (1):442-458. doi: 10.1016/j.ygeno.2019.03.007.

Brukhin V., Osadtchiy J.V., Florez-Rueda A. M., Smetanin D., Nobre M.S., Bakin E., Grossniklaus U. (2019) The Boechera Genus as a Resource for Apomixis Research. Frontiers in Plant Science - Special Issue Plant Breeding, March 2019, v. 10:392. doi: 10.3389/fpls.2019.00392.

Brukhin V., Baskar R. (2019) A brief note on genes that trigger components of apomixis. Journal of Biosciences, 44:45. https://doi.org/10.1007/s12038-019-9850-1.

Kliver S., Rayko M., Komissarov A., Bakin E., Zhernakova D., Prasad K., Rushworth C., Baskar R., Smetanin D., Schmutz J., Rokhsar D.S., Mitchell-Olds T., Grossniklaus U., Brukhin V. (2018) Assembly of the Boechera retrofracta Genome and Evolutionary Analysis of Apomixis-Associated Genes. Genes (Basel), 9(4). pii: E185. doi: 10.3390/genes9040185.

Brukhin V. (2017) Is sex irreplaceable? Towards the molecular regulation of apomixis. Int. J. Plant Reproduct. Biol. 9(2),153-169.

Yurchenko A, Yudin N, Aitnazarov R, Plyusnina A, Brukhin V, Soloshenko V, Lhasaranov B, Popov R, Paronyan IA, Plemyashov KV, Larkin DM. (2017) Genome-wide genotyping uncovers genetic profiles and history of the Russian cattle breeds. Heredity. doi: 10.1038/s41437-017-0024-3.

Osadtchiy J. V., Naumova T. N., Brukhin V. B. (2017) APOMIXIS IN THE GENUS BOECHERA (BRASSICACEAE). Bot. J. 102 (12): 1587-1607.     DOI: 10.1134/S0006813617120018

Brukhin V. (2017) Molecular and Genetic Regulation of Apomixis. Russ J Genetics  53 (9),  1001-1024. DOI: 10.1134/S1022795417090046

Oleksyk TK, Brukhin V, O'Brien SJ. (2015) Putting Russia on the genome map. Science. 13; 350 (6262):747.

Oleksyk TK, Brukhin V, O'Brien SJ. (2015) The Genome Russia project: closing the largest remaining omission on the world Genome map. GigaScience; 4:53. DOI: 10.1186/s13742-015-0095-0

Dobrynin P, Liu S, Tamazian G, Xiong Z, Yurchenko AA, Krasheninnikova K, Kliver S, Schmidt-Küntzel A, Koepfli KP, Johnson W, Kuderna LF, García-Pérez R, Manuel Md, Godinez R, Komissarov A, Makunin A, Brukhin V, Qiu W, Zhou L, Li F, Yi J, Driscoll C, Antunes A, Oleksyk TK, Eizirik E, Perelman P, Roelke M, Wildt D, Diekhans M, Marques-Bonet T, Marker L, Bhak J, Wang J, Zhang G, O'Brien SJ. (2015) Genomic legacy of the African cheetah, Acinonyx jubatus. Genome Biol.; 16:277.

Gaik Tamazian, Serguei Simonov, Joan Pontius, Alexey Makunin, Anton Logachev, Aleksey Komissarov, Nikolay Cherkasov, Andrey Shevchenko, Vladimir Brukhin, Anton Svitin, Pavel Dobrynin, Klaus-Peter Koepfli, Carlos A Driscoll, Kevin Blackistone, Cristina Barr, David Goldman, Agostinho Antunes, Javier Quilez, Belen Lorente-Galdos, Can Alkan, Tomas Marques-Bonet, Marylin Menotti-Raymond, Victor David, Kristina Narfstrom, LaDeana W Hillier, Patrick Minx, Wesley Warren and Stephen J O'Brien (2014). Annotated Features of Domestic Cat-Felis Catus. GigaScience, 3: 13.

Brukhin V. and Morozova N. (2011) Plant growth and development - basic knowledge and current views. Mathematical Modelling Natural Phenomena, v.6, No.2, 1- 53.

Brukhin V., Jaciubek M., Bolanos Carpio, A.., Kuzmina V., Grossniklaus, U. (2011) Female Gametophytic Mutants of Arabidopsis thaliana Identified in a Gene Trap Insertional Mutagenesis Screen. Int. J. Dev. Biol. 55 : 73-84.   

Chekanova J.A., Gregory B.D., Reverdatto S.V., Chen H., Kumar R., Hooker T., Junshi Yazaki J., Li P., Skiba N., Peng Q., Alonso J., Brukhin V., Grossniklaus U., Ecker J.R.; Belostotsky D.A. (2007) Genome-Wide High-Resolution Mapping of Exosome Substrates Reveals Hidden Features in the Arabidopsis Transcriptome. Cell, 131 (7):1340-1353.

Dumbliauskas E., Lechner E., Alioua M., Cognat V., Brukhin V., Berger F., Koncz C., Grossniklaus U., Molinier J., Genschik P. (2011) The Arabidopsis CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEA parental imprinting. EMBO J. 30(4): 731-43.

Brukhin V., Curtis M.D., Grossniklaus U. (2005) The angiosperm female gametophyte: no longer forgotten generation. Review Current Science, 89 (11): 1844-1852.

Brukhin V., Gheyeselinck J., Gagliardini V., Genschik P., Grossniklaus U. (2005) The RPN1 subunit of the 26S proteasome in Arabidopsis is essential for embryogenesis. The Plant Cell, 17 (10): 2723-37.

Thomann A. *, Brukhin V.* , Dieterle M., Gheyeselinck J., Grossniklaus U., Genschik P. (2005) Arabidopsis CUL3A and CUL3B genes are essential for normal embryogenesis. The Plant Journal, 43 (3): 437-448. * These authors contributed equally to this work.

Brukhin V., Hernould M., Gonzalez N., Chevalier C., Mouras A. (2003) Flower development schedule in tomato Lycopersicon esculentum cv. sweet cherry. Sex. Plant Reproduction. 15 (6): 311-320.

Brukhin V.B. (2006) Histochemical and immunohistochemical aspects of embryogenesis. In: Embryology of flowering plants Terminology and Concepts. Ed. Prof. T.Batygina. Enfield, NH, USA, Science  Publisher Inc. vol. 2 Seed, pp. 370-373.

Batygina T.B., Brukhin V.B. (2006) Embryogenesis in Paeoniaceae. In: Embryology of flowering plants. Terminology and Concepts Ed. Prof. T.Batygina. Enfield, NH, USA, Science Publisher  Inc. vol. 2 Seed, pp. 351-355.

Brukhin V., Grossniklaus U. (2003) Identification of genes affecting female gametogenesis: putative role of regulatory subunit of the 26S proteasome in the switch from meiosis to mitosis. Proceed. of the workshop “Embryogenesis and development regulation in plants”, 6-7 March 2003, Turin, Italy.

Grossniklaus, U. Moore, J.M., Brukhin, V., Gheyselinck, J., Baskar, R., Vielle-Calzada, J-P. , Baroux, C., Page, D.R., and Spillane, C. (2002). Engineering of apomixis in crop plants: what can we learn from sexual model systems. In: Vasil IK (Ed) Plant Biotechnology 2002 & Beyond, Kluwer Academic Publishers: Dordrecht, p309-314.

Brukhin V., Clapham D., Elfstrand M., von Arnold S. (2000) Basta tolerance as a selectable and screening marker for transgenic plants of Norway spruce.- Plant Cell Reports, 19: 899-903.

Filonova L.H., Bozhkov P.V., Brukhin V.B., Daniel G., Zhivotovsky B. and von Arnold S. (2000) Two waves of programmed cell death occur during formation and development of somatic embryos in the gymnosperm Norway spruce. J Cell Sci, 113 (24): 4399-4411.

Brukhin V.B., Moleva I.R., Filonova L.H., Grakhov V.P., Blume Ya.B, Bozhkov P.V. (1996)  Proliferative activity of  callus culture of Taxus baccata in relation to anticancer diterpenoid taxol biosynthesis. Biotechnology Letters (Chapman & Hall, London), vol. 18, N 11, pp. 1309-1314.

Brukhin V.B., Bozhkov P.V. (1996) Female gametophyte development and embryogenesis in Taxus baccata L.  – Acta Soc.  Bot.Pol., vol. 65, N 1-2, pp. 135-139.

Tchorzewska D.,  Brukhin V.B., Bednara J. (1996) Organelle layers at meiocyte of Psilotum nudum. - Acta  Soc.Bot.Pol., vol. 65, N 1-2, pp. 91-96.

Brukhin.V., Zemanek A. (1996) The manuscripts of Russian translations of the Polish herbal /Zielnik/ (1613) by Syreniusz in St.Petersburg.- Kwartalnik historii nauki i techniki (Warsaw), vol. 41, N 3-4, pp. 189-195.

Brukhin V.B. (1994) Somatic embryogenesis in vitro and in vivo. - Tranasactions of Vth Conf. of  Botanists in St. Petersburg, St. Petersburg, May 1994 - pp. 97 - 100.

Brukhin V.B., Batygina T.B. (1994) Morphogenesis in culture of peony in vitro.  Biologia Plantarum  supplement, v. 36 - p. 78.

Brukhin V.B., Batygina T.B. (1994) Embryo culture and somatic embryogenesis in culture of Paeonia anomala L. - Phytomorphology. - v. 44, N 3 & 4. - pp. 151-157.

Brukhin V.B. (1993) Some questions of embryogenesis  of  Paeonia L. (Paeoniaceae). - Transactions of IVth Conf. of Botanists, St.Petersburg, Russia, part 2, pp.37-43, VINITI 10.06.93, N 1622-B 93.

Other Essential Publications and Abstracts/ Communications:

Е.А. Бакин , Ф. Сезер, М. П. Райко, К. М. Таскин, В.Б. Брюхин (2020) Филогенетический и функциональный анализ генов, ассоциированных с апомиксисОМ, CENH3 и APOLLO. Актуальная биотехнология. 1-5.

Obrien S.J., Zhernakova D.V., Brukhin V., Malov S., Oleksyk T.K., Koepfli K.P., Zhuk A., Dobrynin P., Kliver S., Cherkasov N., Tamazian G., Rotkevich M., Krasheninnikova K., Evsyukov I., Sidorov S., Gorbunova A., Chernyaeva E., Shevchenko A., Kolchanova S., Komissarov A. et al. (2019) THE GENOME RUSSIA PROJECT-2019. В книге: VII International Congress and Associate Symposiums of Vavilov Society of Geneticists and Breeders on the 100th Anniversary of The Department of Genetics of Saint Petersburg State University. Сборник тезисов Международного Конгресса. 2019. p. 89. eLIBRARY ID: 39156238

Zhernakova D.V., Evsyukov I., Zhuk A., Dobrynin P., Malov S., Cherkasov N., Tamazyan G., Rotkevich M., Krasheninnikova K., Gorbunova A., Shevchenko A., Komissarov A., Simonov S., Logachev A., Novozhilov A., Oleksik T.K., Köpfli K.P., Brukhin V., Obrien S. (2019) THE STRUCTURE OF RUSSIAN POPULATIONS ACCORDING TO THE DATA OF THE PROJECT "RUSSIAN GENOMS". In the book: VII Congress of the Vavilov Society of Geneticists and Breeders, dedicated to the 100th anniversary of the Department of Genetics, St. Petersburg State University, and associated symposia. Collection of abstracts of the International Congress. 2019. 1025. eLIBRARY ID: 39157312

Brukhin V., Kliver S., Rayko M., Komissarov A., Bakin E., Rushworth C., Mitchell-Olds T., Grossniklaus U. (2018) ASSEMBLY AND COMPARATIVE ANALYSIS OF SOME APOMICTIC BOECHERA SPECIES GENOMES. В книге: Molecular Phylogenetics. Contributions to the 5th Moscow International Conference "Molecular Phylogenetics and Biodiversity Biobanking". Eds. A. Troitsky and L. Rusin. 2018. С. 29-30. DOI: 10.30826/MolPhy2018-14  

Brukhin V., Kliver S., Rayko M., Komissarov A., Bakin E., Rushworth C., Mitchell-Olds T., Grossniklaus U. (2018) ASSEMBLY AND ANNOTATION OF GENOMES OF SOME SPECIES FROM THE APOMICTIC GENUS BOECHERA AND EVOLUTIONARY ANALYSIS OF APOMIXIS-ASSOCIATED GENES. В книге: Bioinformatics of Genome Regulation and Structure\Systems Biology (BGRS\SB-2018). The Eleventh International Conference. 2018. С. 154. DOI: 10.18699/BGRSSB-2018-125

Brukhin V., Thomann A., Dieterle, Gheysenlinck J., Genschik P., Grossniklaus U. The role of the ubiquitin/26S proteasome pathway in plant morphogenesis and embryogenesis. Abstr. XII Interantional Conference on Plant Embryology. Krakow, Poland, September 5-7, 2005. Acta Biol. Cracoviensa 2005, vol.47 suppl. 1, p.34.

Brukhin V., Gheysenlinck J., Genschik P., Grossniklaus U. The RPN1 subunit of the 26S proteasome in Arabidopsis is essential for embryogenesis. Abstr. 18 th International Congres on Sexual Plant Reprouction. Beijing, China, August 20-24, 2004- pp. 81-82.

Brukhin V., Gheysenlinck J., Grossniklaus U. Female gametophytic mutants in Arabidopsis. Abstr. 17 th International Congress on Sexual Plant Reprouction. Lublin, Poland, July 9-13, 2002- p. 39.

Brukhin V., Clapham D., Elfstrand M., von Arnold S. Basta resistance as a selectable and screening marker for transgenic Norway spruce. Forest Biotechnology’99. The International Wood Biotechnology Symposium, Oxford, United Kingdom, July 11-16, 1999 – p.22.

 Brukhin V.B., Hernould M., Mouras A. Spatial and temporal expression of Le 8 gene in tomato flowers and fruits. Abstr. 15 th Congress on Sexual Plant Reproduction. Wageningen, The Netherlands, August 1998 - p. 65.

Filonova L., Malysheva L., Grakhov V., Brukhin V., Blume Ya. Genetic and non-genetic control over biosynthesis of taxol in Taxus baccata L.- XVIII Congress of SPPS, Uppsala, Sweden, 12-17 June, 1997, p.192.

Brukhin V.B., Kubagusheva I.F., Bozhkov P.V. A recovery of callus cultures from shoot segments of Taxus spp. - Proceed. 20-th Belgian-Dutch "LOF Symposium" on  Taxol and other Taxanes as New Anti-Cancer Drugs. 1995, November, Leiden, The Netherlands, p.10.

Brukhin V.B., Batygina T.B. The growth regulators' influence on morphogenesis in embryo culture of peony. Abstr. VIII Int. Congress of Plant Tissue and Cell Culture. Firenze, Italy, June 1994, p. 213.

Brukhin V.B. Influence of growth regulators on culture of embryoids from the seeds of Paeonia anomala L. – Abstr. III Conf. of Russian Society of Plant Physiologists. St.Petersburg, Russia, 1993. - p. 268.

Batygina T.B., Titova G.E., Brukhin V.B. Is the embryo in the mature seed of Paeonia sexual? – Abstr. 12 th International Congress on Sexual Plant Reproduction. Columbus Ohio, USA, July 1992 -pp.4-5.