Авторы: Сергеева М.В., Пулькина А.А., Васильев К.А., Романовская-Романько Е.А., Комиссаров А.Б., Кучур О.А., Егоров А.Ю., Цыбалова Л.М., Стукова М.А.
Recombinant viral vectors represent one of the most promising platforms for creating a new generation of vaccines against tuberculosis. We constructed a vaccine candidate based on a cold-adapted influenza vector with a truncated NS1 protein containing an insert of tuberculosis ESAT-6 and Ag85A antigens. The recombinant virus possessed a cold-adapted and temperature-sensitive phenotype and was attenuated for mice when administered intranasally. Immunofluorescent staining and Western blot showed the expression of ESAT-6 protein in MDCK cells infected by recombinant virus. After intranasal administration to mice, the recombinant virus stimulated a specific anti-tuberculosis CD4 + Th1-type response with the formation of polyfunctional antigen-specific T cells.
Keywords: M. tuberculosis antigen; cold-adapted influenza vector; influenza NS1 protein; recombinant virus; vectored vaccine.