Авторы: Кокинос Е.К., Кузьмина Д.О., Кучур О.А., Цымбал С.А., Галочкина А.В., Завирский А.В., Башарин В.А., Штро А.А., Штиль А.А., Духинова М.С.
Abstract
Introduction. THP-1 cell line represents one of the common research models for monocyte/macrophage-mediated inflammation. Aim – to study the properties of THP-1 monocytes activated with bacterial lipopolysaccharides (LPS), influenza virus strain A/Puerto Rico/8/34 (H1N1), and X-ray irradiation. The cell phenotypic and functional profiles were studied with light microscopy, RT-PCR with reverse transcription, and flow cytometry. We determined that HSP90, HPRT, and GAPDH were suitable reference genes for mRNA expression normalization upon LPS, viral, or irradiation exposure, respectively. The gene metabolic markers of THP-1 cell responses to LPS (GAPDH), virus (HSP90, GAPDH), and irradiation (B2M) were determined suggesting the new targets for monocyte profiling in pathological inflammation. Finally, the comparison of pro-inflammatory cytokines and antigen-presenting activities of THP-1 cells showed the differential cytokine expression upon various stimuli. The profiles of metabolic and functional activities can be further used for THP-1 and monocyte comparative analysis and confirm the utility of THP-1 cell line as a model of inflammation.