J. Mater. Chem. B, 2022, DOI: 10.1039/D2TB00582D
Bacterial biofilms stand for the main etiological factor of dental diseases worldwide. At present, toothpaste with bactericidal chemicals as well as abrasive materials are used as preventive care. However, chemicals cause such adverse side effects as antibiotic usage, fluorides, antiseptics drastically reducing the quality of life. At the same time, rational design of bulk toothpaste materials remains absent. In this work, we demonstrated the mechano-bactericidal strategy as antibiotic-free and safe teeth approach for dental biofilm elimination based on the shape-anisotropic CaCO3 (cubic-, stick- and urchin-shaped). As a proof-of-concept, we demonstrated the superior efficiency of the biofilm eradication from ex vivo teeth for urchin- and stick-shaped microparticles on both E. coli (227±32.6% and 215±33%) and S. aureus (210±54.7% and 202±55.5%) compared to spherical particle which employed in conventional toothpastes. These findings will potentially give rise to the development of novel, more safe toothpaste with antibiotic-free bactericidal activity for the prevention of dental diseases.