Authors: Agbadi R. K., Kaukhova I. E., Tarninko I. I., Sirichenko T.I.
Amino acids and chemical elements play a lead role in all physiological processes in the human body. The consistency of their composition is one of the most important and critical conditions for normal functioning and development of organisms. The variation in composition of amino acids and elements in the body leads to deterioration in health status. One possibility of timely correction of violations of elemental and amino acid homeostasis in the human body is the use of extracts from medicinal plants. Morinda citrifolia, commonly known as Noni, contains essential amino acids for life. Methods: A total of fourteen essential, conditionally essential and nonessential amino acids were examined by thin-layer chromatography (TLC). Two categories of extraction solutions; aqueous extraction and 1% HCl extraction, were prepared. Total quantity of amino acids in the fruits, leaves and roots of noni were investigated by spectrophotometric method of analysis with glutamic acid solution prepared as a standard. Result: The TLC analysis showed presence and absence of amino acids in the parts investigated. The leaves extracts of noni showed the highest content of amino acids whereas the roots demonstrated the least amino acid content. Methionine, an important ingredient for tissue development and growth were found in the 1% HCl sample extraction analyzed with the leaves and roots of noni. Leucine, a muscle building compound was observed in the fruits of the aqueous sample extraction of noni. Total content of amino acids in alcohol extraction from the roots, leaves and fruits of crude extraction of Morinda citrifolia was much lower than in aqueous extractions. Conclusion: This study confirmed the presence of amino acids in noni. Higher total content of amino acids was observed in aqueous medium as compared to ethanol medium. In conclusion, therefore, aqueous extraction of noni presents an optimal amount of amino acids which play crucial antioxidative roles in living organisms.