Nanoreactors Based on DNAzyme-Functionalized Magnetic Nanoparticles Activated by Magnetic Field

Autors: Колпащиков Д.М., S.F. Bakshi, N. Guz, A. Zakharchenko, H. Deng, A. Tumanov, C.D. Woodworth, S. Minko, E. Katz

Abstract

A new biomimetic nanoreactor design, MaBiDz, is presented based on a copolymer brush in combination with superparamagnetic nanoparticles. This cellular nanoreactor features two species of magnetic particles, each functionalized with two components of a binary deoxyribozyme system. In the presence of a target mRNA analyte and a magnetic field, the nanoreactor is assembled to form a biocompartment enclosed by the polymeric brush that enables catalytic function of the binary deoxyribozyme with enhanced kinetics. MaBiDz was demonstrated here as a cellular sensor for rapid detection and imaging of a target mRNA biomarker for metastatic breast cancer, and its function shows potential to be expanded as a biomimetic organelle that can downregulate the activity of a target mRNA biomarker.

DOI: 10.1039/C7NR08581H

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http://pubs.rsc.org/en/content/articlelanding/2018/nr/c7nr08581h#!divAbstract